Terminator
Term667L

Part:BBa_K2760007:Design

Designed by: Diana Isabel Tamayo Ramos   Group: iGEM18_TecMonterrey_GDL   (2018-10-09)


Terminator 667 for Lactobacillus spp.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was designed for Lactobacillus species, synthesis may be troublesome due to the loop sequences.


Source

The terminator sequence was taken from Duong et. al, (2011) [1]. It can also be found in plasmid repositories such as Addgene [2].

References

[1] Duong, T. , Miller, M. J., Barrangou, R. , Azcarate‐Peril, M. A. and Klaenhammer, T. R. (2011), Construction of vectors for inducible and constitutive gene expression in Lactobacillus. Microbial Biotechnology, 4: 357-367. doi:10.1111/j.1751-7915.2010.00200.x

[2] pTR892 (2017) Addgene. Retrieved from: https://www.addgene.org/71803/