Terminator
Term667L
Part:BBa_K2760007:Design
Designed by: Diana Isabel Tamayo Ramos Group: iGEM18_TecMonterrey_GDL (2018-10-09)
Terminator 667 for Lactobacillus spp.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It was designed for Lactobacillus species, synthesis may be troublesome due to the loop sequences.
Source
The terminator sequence was taken from Duong et. al, (2011) [1]. It can also be found in plasmid repositories such as Addgene [2].
References
[1] Duong, T. , Miller, M. J., Barrangou, R. , Azcarate‐Peril, M. A. and Klaenhammer, T. R. (2011), Construction of vectors for inducible and constitutive gene expression in Lactobacillus. Microbial Biotechnology, 4: 357-367. doi:10.1111/j.1751-7915.2010.00200.x
[2] pTR892 (2017) Addgene. Retrieved from: https://www.addgene.org/71803/